Genomic Profiling of Enterobacter mori isolated from apple and potato: Enhancing shelf life against Enterobacter mori using algal oil emulsion based chitosan hydrogel

Abstract

This research involves isolation and growth of bacteria from rotten apples and potatoes. The whole genome sequencing revealed that Enterobacter mori was the cause of apples and potatoes rotting. Genomic profiling and comparative genome analysis of E.mori was carried out, which includes identification of antimicrobial resistance genes, virulence genes, prophage regions, genomic islands and phylogenetic analysis. . The strain of dictyospaerium DHSYM was used to extract algae oil, which was then utilized in the production of algal oil emulsion. After that, this emulsion was incorporated into chitosan hydrogel, and the chitosan hydrogel was coated over apples and potatoes to extend the shelf life of these products and reduce the amount of post-harvest loss. For characterizing, algal oil emulsion based chitosan hydrogel underwent a variety of analyses. Such as scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), Raman spectroscopy (RAMAN), testing for biofilm eradication, antibiotic activity, hemolytic assay, cytotoxicity study, and molecular docking.