Abstract:
Because of their potential in drug delivery, imaging, biosensing, and diverse structural features,
graphene-based nanomaterials have emerged as a prominent area of nanotechnology study,
especially for biomedical applications. For producing these nanomaterials, the green reduction
approach is the most promising as it is a cost-effective and environment-friendly approach that
uses natural extracts and reduces toxic gas production compared to traditional methods. For this
study, we have used the same green reduction method using Cinnamaldehyde and Eugenol to
create reduced graphene oxide/ silver nanoparticles nanohybrids. The synthesized nanohybrids
were characterized by using UV-Vis Spectroscopy, Fourier Transform Infrared spectroscopy,
scanning electron microscopy, X-ray diffraction, Zeta-potential analysis, atomic force
microscopy which confirmed the formation and stability of the well dispersed AgNPs on rGO
surface. The double-stranded and single-stranded binding activity of these nanohybrids was
evaluated using spectroscopy analysis which showed a shift in the absorption wavelength
indicating strong interaction with DNA. Additionally, physiochemical testing assessed the
stability and life span, supporting these nanohybrids’ potential for long-term biomedical
applications. These findings highlight the suitability of these nanohybrids for sustainable
biomedical applications, particularly for DNA binding. With further implications, these can be
utilized in emerging drug delivery, biosensing, and diagnostic sectors.
