Abstract:
Fowl Adenoviruses are very diverse group of pathogens causing diseases like HPS,
IBH, and gizzard erosion in wild and domestic birds. It causes huge economic losses in
poultry all over the world due to its high pathogenicity. Hexon gene is very important to
understand the epidemiological status and genetic relation of FAdV strains. Hexon protein
being main and most abundant capsid protein elicit strong immune response. Various
vaccines are available for protection against fowl adenovirus. Several detection methods like
PCR, HRM curve analysis, AGID test and ELISA etc. are used. Due to high specificity and
high sensitivity, ELISA is mainly used for virus detection. In current study efforts were made
to make ELISA kit for Pakistani poultry samples of fowl adenovirus. Hexon gene was also
isolated from Pakistani sample and protein expressed through that gene was used to coat on
ELISA plate for successful detection of virus present in Pakistani poultry samples. This kit
will help the farmers to detect virus in their flocks at low costs in easy access and in
ecofriendly environment rather than using costly kits available internationally.
