Abstract:
Oil-in-water emulsions are finding increasing scope as delivery systems to encapsulate
lipophilic bioactive components in functional food, personal care, and pharmaceutical
products. Toxicity related issues with simple Amphotericin B (AmB) preparations and
economic issues related to liposomal preparations led to the need of developing cost
effective, nontoxic and therapeutically effective Amphotericin B formulation. Oil in
water emulsion of Amphotericin B was prepared using Hydroxypropylmethylcellulose
(HPMC) polymer, canola oil and tween 80 as an emulsifier. This study aims for the
preparation of AmB formulations, O/W emulsion utilizing HPMC and Carbopol
viscosity enhancing agent to improve the stability of globules produced during the
emulsification process. Influence of various parameters that include surfactant
concentration, polymer concentration, stirring speed and stirring time, on the particle
size of Amphotericin B-loaded emulsions and hence on stability of emulsions was
investigated. Emulsion were prepared based on above parameters were subjected to
various characterization techniques to understand the trend of change in particle size of
emulsion and the particle size distribution. The size, shape and elemental composition
of particles were analysed using particle size distribution analyser (PSD), scanning
electron microscopy (SEM), energy dispersive X-Ray Spectrometry (EDS), FTIR
(Fourier Transform IR Spectroscopy) analysis, zeta Potential Measurement and optical
microscopy. The prepared Amphotericin B emulsions showed the antifungal activity
against several fungus including Aspergillus Tubingensis, Aspergillus Flavus,
Aspergillus Niger when tested. The statistical analysis was also performed for every
individual parameter under study.
