Synthesis, Characterization and Antimicrobial activity of Silver Nanoparticles by Green synthesis

Abstract

Green synthesis of metallic nanoparticles has been developed as environment friendly and cost effective alternative over other methods such as physical and chemical, thus gaining significant attraction in nanomedicine. In present study silver nanoparticles have been synthesized using silver nitrate and various concentrations of methanolic root extract of Rhazya stricta. The color change (yellowish to orange brown) in reaction mixture was observed during synthesis process. Stability and dispersion of nanoparticles was improved by adding xylitol. Synthesized nanoparticles were confirmed using UV–vis spectroscopy, scanning electron microscopy (SEM), Energy dispersive spectroscopy (EDS), X-ray diffractometer (XRD), and Fourier transforms infrared spectroscopy (FTIR). Furthermore, the antibacterial activity was determined against strains of gram positive (Bacillus subtilis) and gram negative (Escherichia coli) bacteria for crude plant extract and synthesized nanoparticles.