Fabrication And Characterization Of Lallemantia royleana Seeds Mucilage (LSM) Based Film For Wound Healing

Abstract

In the present study mucilage forming ability of Lallemantia royleana seeds was utilized for biomedical applications. Phytochemical screening was done to know about the chemical constituents of the mucilage. Antibacterial activity of Lallementia royleana seeds mucilage (LSM) showed good positive results when tested against. LSM showed good positive results when tested against Bordetella pertussis (B. pertussis), Salmonella typhimurium (S. typhimurium), Shigella dysenteriae (S. dysenteriae) and Klebsiella Pneumoniae (K. pneumonia). Among all extracts distilled water extract show best results while Hexane extract show poor activity as compared to standard antibiotics. LSM was used to fabricate biodegradable film for wound healing by combining homeostatic effect of sodium alginate, plasticizing property of glycerol and therapeutic capabilities of LSM. Furthermore, various tests were performed for characterizations of the film. Mechanical testing was done to evaluate physical properties. Results showed that LSM concentration greatly affects the properties of the film. Film with 70% LSM has more brittle nature as compared to films with 50% and 60%concentration. The tensile properties decrease with decreasing LSM concentration. While the percentage elongation increases as the concentration was decreased to 50%. Scanning electron microscopy was done for surface characterization. Film with 70% LSM was more porus and brittle as compared to film with 60% and 50% LSM. To test the hydrophilicity and in vitro degradation properties films were immersed in PBS solution and were checked at regular intervals for. It was found that increasing the LSM concentration greatly increases the water absorption ability of the film. It was found that film with the highest concentration of LSM lose weight much quicker as compared to film with lowest LSM.