Zinc Oxide Nanoparticles Inhibit Human Laryngeal Carcinoma by Regulating Redox Status

Abstract

Nanotechnology research has gained momentum in the recent years by providing innovative solutions in the field of biomedical, materials science, optics and electronics. Nanoparticles are essentially a varied form of basic elements derived by altering their atomic and molecular properties of elements. This article elaborates on the properties and applications of zinc oxide nanoparticles. Main interest in the nanotechnology permits is the controlled synthesis of materials and the Nano size of the particle which is notably smaller than most of the cells in human body. The ultra-small size is equivalent to proteins naturally present in our body. Nanoparticles applications are used in many fields such as cancer treatment. For the treatment of laryngeal cancer zinc oxide nano particles are aimed to use. The nanoparticles are synthesized, and laryngeal carcinoma is treated followed by protein expression and anti-oxidant activity determination. Genetically mutated genes in laryngeal carcinoma reportedly are caspase 3, actin, BCL2 and PARP. Caspase 3 (cysteine-aspartic acid protease) is an apoptotic protein that interacts with caspase 8 and caspase 9 to initiate apoptosis in the damaged cells. It is encoded by caspase 3 gene. Because of mutation in this gene the damaged cells remain and grow causing the cancer. In the process of cancer invasion and metastasis actin cytoskeleton and focal adhesion are involved. For the intensification the metastatic process actin proteins are involved along with then the actin binding proteins such as cofilin and N-WASP and the focal adhesion proteins such as α- and β-Parvin and PINCH are reportedly involved. They support the cell adhesion, migration, survival and regulation of actin cytoskeleton dynamics in laryngeal cancer. Our results suggest that treatment with optimized doses of zinc oxide nanoparticles up regulates PARP and Caspase-3 and down regulates Bcl-2, thus proving effective in cancer cells therapy. Key words: Caspase-3, PARP, Bcl-2, apoptosis, mutation, metastasis, momentum, proliferation, therapeutic potential