Analysis of the Efficacy of Novel Drug Based on Natural Plant Extract for the Treatment of Type II Diabetes

Abstract

Type 2 diabetes is the most common form of diabetes mellitus. The aim of this study was to develop and standardize the polyhedral formulation (granule) and checked its efficacy with regards to type 2 diabetes. The alcoholic extract of each plant (H. antidysentrica, Prunus dulcis and Cicer arietinum) and oleic acid was mixed and then formulated by wet granulation method. FTIR was done to investigate the presence of active compounds. Physicochemical properties of granules were evaluated, and antidiabetic potential was substantiated through inhibition of carbohydrate digestive enzyme such as α- amylase and α-glucosidase, glucose uptake activity in yeast cells and antioxidant activity. IR spectra indicated the presence of active compounds such as phenols and amines and showed the absence of interaction between drug and excipients. The dissolution profile of active pharmaceutical ingredient (API) from granules showed more 72–80% release in 2hrs. Granules exhibited better inhibition of ɑ-amylase and ɑ-glucosidase as on comparison with the standard drug and found to be dose dependent. The enhanced uptake of glucose was observed with decrease in drug concentration. Moreover, the DPPH scavenging activity was high (98%) at 1mg/ml. The stabilized formulation (granules) was formed and the presence of active compounds is responsible for the better antidiabetic activity by inhibiting carbohydrate digesting enzymes. Hence, it could lower the post prandial hyperglycemia and has the potential to use for the treatment of type II diabetes prior to determine the dose regime.