Abstract:
The increasing prevalence of surgical site infections and the demand for advanced wound care
solutions have driven the development of innovative biomaterials. This study investigates the
quantification of Indica /Ch composite layered silk braided stitches for enhancing tissue repairing
in wounds and reducing infections. Sutures were coated with Azadirachta Indica extract in
chitosan, and their antibacterial, hemocompatibility, and wound healing properties were
evaluated. The coated stitches demonstrated antibacterial activity against Aureus and E. coli, with
the satisfactory zone of inhibition observed. Hemolysis assays revealed minimal hemolytic
activity (<2%), confirming the safety of the coated sutures for clinical use. In vivo studies using
Sprague Dawley rats showed that wounds closed with \ Indica/Ch-coated stitches exhibited
reduced inflammation, faster healing, and complete recovery within Two weeks. In contrast,
uncoated sutures resulted in persistent inflammation and delayed healing. Scanning Electron
Microscopy (SEM) analysis confirmed the uniformity and smoothness of the coating, further
supporting its structural integrity. These findings highlight the potential of Azadirachta
Indica/chitosan-coated sutures as a safe, effective, and biocompatible solution for surgical wound
care. By combining the antimicrobial and antioxidant properties of Azadirachta Indica with the
bio adhesive nature of chitosan, this study demonstrates a promising advancement in the
development of bioactive suture materials. This research underscores the importance of
integrating traditional medicinal knowledge with modern biomedical technologies to address
critical healthcare challenges and improve patient outcomes.
