Coated Suture To Enhance Self-Healing And Hemocompatibility For Wound Healing Application

Abstract

The increasing prevalence of surgical site infections and the demand for advanced wound care solutions have driven the development of innovative biomaterials. This study investigates the quantification of Indica /Ch composite layered silk braided stitches for enhancing tissue repairing in wounds and reducing infections. Sutures were coated with Azadirachta Indica extract in chitosan, and their antibacterial, hemocompatibility, and wound healing properties were evaluated. The coated stitches demonstrated antibacterial activity against Aureus and E. coli, with the satisfactory zone of inhibition observed. Hemolysis assays revealed minimal hemolytic activity (<2%), confirming the safety of the coated sutures for clinical use. In vivo studies using Sprague Dawley rats showed that wounds closed with \ Indica/Ch-coated stitches exhibited reduced inflammation, faster healing, and complete recovery within Two weeks. In contrast, uncoated sutures resulted in persistent inflammation and delayed healing. Scanning Electron Microscopy (SEM) analysis confirmed the uniformity and smoothness of the coating, further supporting its structural integrity. These findings highlight the potential of Azadirachta Indica/chitosan-coated sutures as a safe, effective, and biocompatible solution for surgical wound care. By combining the antimicrobial and antioxidant properties of Azadirachta Indica with the bio adhesive nature of chitosan, this study demonstrates a promising advancement in the development of bioactive suture materials. This research underscores the importance of integrating traditional medicinal knowledge with modern biomedical technologies to address critical healthcare challenges and improve patient outcomes.