Abstract:
The production of pharmacological products from medicinal plants have now become one of
the leading cause of economic development of countries. Currently, the purification of crude
extract and further isolation of bioactive compounds from medicinal plants are gaining special
attention by the researchers to obtain natural bioactive formulations. Therefore, to overcome
the phenomena of multidrug resistance, the method of using natural products and drugs seems
to be an effective strategy against different diseases. This study is carried out to investigate
the bioactive flavonoids of Berberis lycium leaves on Escherichia coli. A 15 % methanol in
ethyl acetate as a mobile phase was selected by thin layer chromatography that gave better
separation of flavonoids. After the collection of two seventy- six fractions from column
chromatography, they were combined based on similar Rf values. Hence thirteen fractions
were made. Out of thirteen fractions, five fractions having single spots were selected for
antibacterial test against six bacterial strains that included three-gram positive bacteria:
Enterococcus faecalis, Bacillus cereus, Staphylococcus aureus, and three-gram negative
bacteria: Salmonella typhi, Pseudomonas aeruginosa, Escherichia coli. The separated
fractions showed effective antibacterial activity against two bacterial strains; P. aeruginosa,
and E. coli. The characterization was done by FTIR analysis for further confirmation of groups
belong to flavonoids in isolated samples.
