Isolation and Characterization of Flavonoids from Berberis lycium Leaves

Abstract

The production of pharmacological products from medicinal plants have now become one of the leading cause of economic development of countries. Currently, the purification of crude extract and further isolation of bioactive compounds from medicinal plants are gaining special attention by the researchers to obtain natural bioactive formulations. Therefore, to overcome the phenomena of multidrug resistance, the method of using natural products and drugs seems to be an effective strategy against different diseases. This study is carried out to investigate the bioactive flavonoids of Berberis lycium leaves on Escherichia coli. A 15 % methanol in ethyl acetate as a mobile phase was selected by thin layer chromatography that gave better separation of flavonoids. After the collection of two seventy- six fractions from column chromatography, they were combined based on similar Rf values. Hence thirteen fractions were made. Out of thirteen fractions, five fractions having single spots were selected for antibacterial test against six bacterial strains that included three-gram positive bacteria: Enterococcus faecalis, Bacillus cereus, Staphylococcus aureus, and three-gram negative bacteria: Salmonella typhi, Pseudomonas aeruginosa, Escherichia coli. The separated fractions showed effective antibacterial activity against two bacterial strains; P. aeruginosa, and E. coli. The characterization was done by FTIR analysis for further confirmation of groups belong to flavonoids in isolated samples.